Nucleolar specific acidic phosphoprotein C23 is highly methylated.
نویسندگان
چکیده
منابع مشابه
Antibodies to a nucleolar protein are localized in the nucleolus after red blood cell-mediated microinjection
To determine whether red blood cell-mediated microinjection of antibodies can be used to study nuclear protein localization and function, we microinjected antibodies that have been shown to react specifically with nucleolar acidic phosphoprotein C23 into Walker 256 cells. The intracellular distribution of microinjected anti-C23 antibodies and preimmune immunoglobulins were determined by immunof...
متن کاملIdentification and cDNA cloning of a Xenopus nucleolar phosphoprotein, xNopp180, that is the homolog of the rat nucleolar protein Nopp140.
The monoclonal antibody G1C7, recognises both Xenopus nucleolin and a protein of 180 kDa present in Xenopus oocyte nucleoli. This antibody was used to obtain a cDNA clone encoding the 180 kDa protein now called xNopp180 (Xenopus nucleolar phosphoprotein of 180 kDa). Analysis of the deduced amino acid sequence from this cDNA shows that xNopp180 is almost entirely composed of alternating acidic a...
متن کاملcDNA sequences of chicken nucleolin/C23 and NO38/B23, two major nucleolar proteins.
Nucleolin and NO38 are abundant nucleolar proteins implicated in the transcription and processing of ribosomal RNA, and in the packaging and transport of ribosomal proteins or preribosomal particles (for references see 1-5). cDNAs encoding the chicken homologs of these proteins were cloned (by lmmunoscreening; 6) from Xgtll cDNA expression libraries prepared from poly(A)+ RNA of 10 day old chic...
متن کاملNucleolar proteins B23 and C23 as target antigens in chronic graft-versus-host disease.
Previously we observed that sera from recipients of allogeneic bone marrow transplants who developed extensive chronic graft-versus-host disease (GVHD) intensively stained the nucleolar region of target cells in indirect immunofluorescence microscopy. To identify the target antigens, immunoblotting experiments were performed using isolated nuclei, isolated nucleoli, and purified nuclear and nuc...
متن کاملProtein kinase CK2 is inhibited by human nucleolar phosphoprotein p140 in an inositol hexakisphosphate-dependent manner.
Protein kinase CK2 is a ubiquitous protein kinase that can phosphorylate various proteins involved in central cellular processes, such as signal transduction, cell division, and proliferation. We have shown that the human nucleolar phosphoprotein p140 (hNopp140) is able to regulate the catalytic activity of CK2. Unphosphorylated hNopp140 and phospho-hNopp140 bind to the regulatory and catalytic...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)33317-9